Evidence for a Cytolytic Factor Released by Macrophages
نویسندگان
چکیده
Mouse peritoneal macrophages cultivated in vitro acquire a strong extracellular cytotoxic activity towards isotope labeled syngeneic erythrocytes as demonstrated by isotope release to the medium. This lytic process is mediated by an extremely labile macrophage cytolytic factor (MCF) which is not detected under ordinary tissue culture conditions with serum present in the medium. By the use of serum-free medium containing low doses of 2-mercaptoethanol MCF is stabilized and found to be an easily dialysable, low molecular substance which resists heating at 60 degrees C for 30 min.
منابع مشابه
Production of a tumor cytolytic factor(s) by activated human alveolar macrophages and its action.
When human alveolar macrophages (AM) lavaged from healthy donors were incubated in medium with or without lipopolysaccharide (LPS) or muramyl dipeptide, they released a factor(s) responsible for tumor cell killing. The activity of the tumor cytolytic factor(s), called TCF, was determined by radioactive release assay. Human AM released variable amounts of TCF into the culture medium without any ...
متن کاملInterferonlike factors from antigen- and mitogen-stimulated human leukocytes with antirickettsial and cytolytic actions on Rickettsia prowazekii. Infected human endothelial cells, fibroblasts, and macrophages
Unique features of the primary site of rickettsial replication in typhus fevers, i.e., within the endothelial cells of small blood vessels in tissues, suggest that effector mechanisms, other than those dependent on phagocytosis by activated macrophages with enhanced microbicidal properties, most likely are necessary to explain the cell-mediated immune control of intracellular rickettsial replic...
متن کاملTumoricidal Effector Mechanisms of Murine Bacillus Calmette-Guerin-SLCtivated Macrophages: Mediation of Cytolysis, Mitochondrial Respiration Inhibition, and Release of Intracellular Iron by Distinct Mechanisms1
Murine Bacillus Ca/mette-Gumn-activated macrophages mediate dis crete cytotoxic effects in cocultured tumor target cells in vitro. These effects include: (a) the loss of intracellular iron, in part associated with reversible inhibition of the Kreb's cycle enzyme, aconitase; (A)cytostasis, associated with reversible lesions inflicted in the electron transpon chain (ETC) of the mitochondria resul...
متن کاملMechanisms of neutrophil accumulation in the lungs of patients with idiopathic pulmonary fibrosis.
Neutrophils are a characteristic feature of the alveolitis of idiopathic pulmonary fibrosis (IPF). a chronic disorder limited to lung. One mechanism by which neutrophils may be selectively attracted to lung and not other tissues is via the secretion of the neutrophil-specific chemotactic factor by alveolar macrophages. To evaluate the role of alveolar macrophages in modulating the migration of ...
متن کاملRole of macrophage oxidative burst in the action of anthrax lethal toxin.
BACKGROUND Major symptoms and death from systemic Bacillus anthracis infections are mediated by the action of the pathogen's lethal toxin on host macrophages. High levels of the toxin are cytolytic to macrophages, whereas low levels stimulate these cells to produce cytokines (interleukin-1 beta and tumor necrosis factor-alpha), which induce systemic shock and death. MATERIALS AND METHODS Expe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 140 شماره
صفحات -
تاریخ انتشار 1974